gdf5 antibody a 10 (Santa Cruz Biotechnology)
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Gdf5 Antibody A 10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gdf5+antibody+a+10/GDF-5+Antibody/pmc06631233-205-6-10
Average 93 stars, based on 15 article reviews
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1) Product Images from "Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle"
Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle
Journal: Non-Coding RNA
doi: 10.3390/ncrna5020033
Figure Legend Snippet: Knockdown of Myoparr altered global gene expressions in denervated skeletal muscle. The heatmap displayed the changes in expression for 848 genes altered by Myoparr knockdown in denervated TA muscle of C57BL/6J mice (values are z-scores). The black and red arrow indicate the decreased expression of myogenin and increased expression of Gdf5 , respectively.
Techniques Used: Knockdown, Expressing
Figure Legend Snippet: Upregulated genes encoding secretory proteins following Myoparr knockdown in denervated tibialis anterior (TA) muscles of C57BL/6J mice.
Techniques Used: Knockdown, Muscles
Figure Legend Snippet: Myoparr knockdown activated bone morphogenetic protein (BMP) signaling through the expression of growth/differentiation factor 5 (GDF5) in denervated TA muscles of C57BL/6J mice. ( a ) Western blot showing increased expression of GDF5 by Myoparr knockdown in TA muscles 3 d post denervation. The activity of BMP signaling was indicated by the levels of phosphorylated Smad1/5/8 (pSmad1/5/8). GAPDH expression served as an internal control. Three independent experiments were shown. ( b ) Box-and-whisker plots showed relative quantification of GDF5 expression as GDF5/GAPDH ratio ( n = 3 per group). * p < 0.05, unpaired two-tailed Student’s t -test. ( c ) Relative quantification of the activity of BMP signaling was shown as pSmad1/5/8/Smad5 ratio ( n = 3 per group).
Techniques Used: Knockdown, Expressing, Muscles, Western Blot, Activity Assay, Control, Whisker Assay, Quantitative Proteomics, Two Tailed Test
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Knockdown:Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody Expressing:Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody Muscles:Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody Western Blot:Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody Activity Assay:Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody Control:Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody Whisker Assay:Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody Quantitative Proteomics:Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody Two Tailed Test:Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody |