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gdf5 antibody a 10  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology gdf5 antibody a 10
    Knockdown of Myoparr altered global gene expressions in denervated skeletal muscle. The heatmap displayed the changes in expression for 848 genes altered by Myoparr knockdown in denervated TA muscle of C57BL/6J mice (values are z-scores). The black and red arrow indicate the decreased expression of myogenin and increased expression of <t>Gdf5</t> , respectively.
    Gdf5 Antibody A 10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gdf5+antibody+a+10/GDF-5+Antibody/pmc06631233-205-6-10
    Average 93 stars, based on 15 article reviews
    gdf5 antibody a 10 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle"

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle

    Journal: Non-Coding RNA

    doi: 10.3390/ncrna5020033

    Knockdown of Myoparr altered global gene expressions in denervated skeletal muscle. The heatmap displayed the changes in expression for 848 genes altered by Myoparr knockdown in denervated TA muscle of C57BL/6J mice (values are z-scores). The black and red arrow indicate the decreased expression of myogenin and increased expression of Gdf5 , respectively.
    Figure Legend Snippet: Knockdown of Myoparr altered global gene expressions in denervated skeletal muscle. The heatmap displayed the changes in expression for 848 genes altered by Myoparr knockdown in denervated TA muscle of C57BL/6J mice (values are z-scores). The black and red arrow indicate the decreased expression of myogenin and increased expression of Gdf5 , respectively.

    Techniques Used: Knockdown, Expressing

    Upregulated genes encoding secretory proteins following Myoparr knockdown in denervated tibialis anterior (TA) muscles of C57BL/6J mice.
    Figure Legend Snippet: Upregulated genes encoding secretory proteins following Myoparr knockdown in denervated tibialis anterior (TA) muscles of C57BL/6J mice.

    Techniques Used: Knockdown, Muscles

    Myoparr knockdown activated bone morphogenetic protein (BMP) signaling through the expression of growth/differentiation factor 5 (GDF5) in denervated TA muscles of C57BL/6J mice. ( a ) Western blot showing increased expression of GDF5 by Myoparr knockdown in TA muscles 3 d post denervation. The activity of BMP signaling was indicated by the levels of phosphorylated Smad1/5/8 (pSmad1/5/8). GAPDH expression served as an internal control. Three independent experiments were shown. ( b ) Box-and-whisker plots showed relative quantification of GDF5 expression as GDF5/GAPDH ratio ( n = 3 per group). * p < 0.05, unpaired two-tailed Student’s t -test. ( c ) Relative quantification of the activity of BMP signaling was shown as pSmad1/5/8/Smad5 ratio ( n = 3 per group).
    Figure Legend Snippet: Myoparr knockdown activated bone morphogenetic protein (BMP) signaling through the expression of growth/differentiation factor 5 (GDF5) in denervated TA muscles of C57BL/6J mice. ( a ) Western blot showing increased expression of GDF5 by Myoparr knockdown in TA muscles 3 d post denervation. The activity of BMP signaling was indicated by the levels of phosphorylated Smad1/5/8 (pSmad1/5/8). GAPDH expression served as an internal control. Three independent experiments were shown. ( b ) Box-and-whisker plots showed relative quantification of GDF5 expression as GDF5/GAPDH ratio ( n = 3 per group). * p < 0.05, unpaired two-tailed Student’s t -test. ( c ) Relative quantification of the activity of BMP signaling was shown as pSmad1/5/8/Smad5 ratio ( n = 3 per group).

    Techniques Used: Knockdown, Expressing, Muscles, Western Blot, Activity Assay, Control, Whisker Assay, Quantitative Proteomics, Two Tailed Test

    Related Articles

    Knockdown:

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle
    Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).. The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.

    Expressing:

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle
    Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).. The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.

    Muscles:

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle
    Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).. The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.

    Western Blot:

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle
    Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).. The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.

    Activity Assay:

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle
    Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).. The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.

    Control:

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle
    Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).. The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.

    Whisker Assay:

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle
    Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).. The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.

    Quantitative Proteomics:

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle
    Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).. The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.

    Two Tailed Test:

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle
    Article Snippet: Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.Equal amounts of protein quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) were subjected to western blot analysis.. The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).. The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.The horseradish peroxidase (HRP)-linked secondary antibodies included an anti-mouse IgG (#7076, Cell Signaling Technology) and an anti-rabbit IgG (#7074, Cell Signaling Technology) as appropriate.



    Similar Products

    93
    Santa Cruz Biotechnology gdf5 antibody a 10
    Knockdown of Myoparr altered global gene expressions in denervated skeletal muscle. The heatmap displayed the changes in expression for 848 genes altered by Myoparr knockdown in denervated TA muscle of C57BL/6J mice (values are z-scores). The black and red arrow indicate the decreased expression of myogenin and increased expression of <t>Gdf5</t> , respectively.
    Gdf5 Antibody A 10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gdf5+antibody+a+10/GDF-5+Antibody/pmc06631233-205-6-10
    Average 93 stars, based on 1 article reviews
    gdf5 antibody a 10 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    Knockdown of Myoparr altered global gene expressions in denervated skeletal muscle. The heatmap displayed the changes in expression for 848 genes altered by Myoparr knockdown in denervated TA muscle of C57BL/6J mice (values are z-scores). The black and red arrow indicate the decreased expression of myogenin and increased expression of Gdf5 , respectively.

    Journal: Non-Coding RNA

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle

    doi: 10.3390/ncrna5020033

    Figure Lengend Snippet: Knockdown of Myoparr altered global gene expressions in denervated skeletal muscle. The heatmap displayed the changes in expression for 848 genes altered by Myoparr knockdown in denervated TA muscle of C57BL/6J mice (values are z-scores). The black and red arrow indicate the decreased expression of myogenin and increased expression of Gdf5 , respectively.

    Article Snippet: The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).

    Techniques: Knockdown, Expressing

    Upregulated genes encoding secretory proteins following Myoparr knockdown in denervated tibialis anterior (TA) muscles of C57BL/6J mice.

    Journal: Non-Coding RNA

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle

    doi: 10.3390/ncrna5020033

    Figure Lengend Snippet: Upregulated genes encoding secretory proteins following Myoparr knockdown in denervated tibialis anterior (TA) muscles of C57BL/6J mice.

    Article Snippet: The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).

    Techniques: Knockdown, Muscles

    Myoparr knockdown activated bone morphogenetic protein (BMP) signaling through the expression of growth/differentiation factor 5 (GDF5) in denervated TA muscles of C57BL/6J mice. ( a ) Western blot showing increased expression of GDF5 by Myoparr knockdown in TA muscles 3 d post denervation. The activity of BMP signaling was indicated by the levels of phosphorylated Smad1/5/8 (pSmad1/5/8). GAPDH expression served as an internal control. Three independent experiments were shown. ( b ) Box-and-whisker plots showed relative quantification of GDF5 expression as GDF5/GAPDH ratio ( n = 3 per group). * p < 0.05, unpaired two-tailed Student’s t -test. ( c ) Relative quantification of the activity of BMP signaling was shown as pSmad1/5/8/Smad5 ratio ( n = 3 per group).

    Journal: Non-Coding RNA

    Article Title: Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle

    doi: 10.3390/ncrna5020033

    Figure Lengend Snippet: Myoparr knockdown activated bone morphogenetic protein (BMP) signaling through the expression of growth/differentiation factor 5 (GDF5) in denervated TA muscles of C57BL/6J mice. ( a ) Western blot showing increased expression of GDF5 by Myoparr knockdown in TA muscles 3 d post denervation. The activity of BMP signaling was indicated by the levels of phosphorylated Smad1/5/8 (pSmad1/5/8). GAPDH expression served as an internal control. Three independent experiments were shown. ( b ) Box-and-whisker plots showed relative quantification of GDF5 expression as GDF5/GAPDH ratio ( n = 3 per group). * p < 0.05, unpaired two-tailed Student’s t -test. ( c ) Relative quantification of the activity of BMP signaling was shown as pSmad1/5/8/Smad5 ratio ( n = 3 per group).

    Article Snippet: The primary antibodies used included the GDF5 antibody A-10 (sc-373744, Santa Cruz Biotechnology, Dallas, TX, USA), the Smad5 antibody YY-6 (sc-101151, Santa Cruz Biotechnology), a phospho-Smad1(Ser463/465)/5(Ser463/465)/8(Ser426/428) antibody (#9511, Cell Signaling Technology, Danvers, MA, USA), and a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#2118, Cell Signaling Technology).

    Techniques: Knockdown, Expressing, Muscles, Western Blot, Activity Assay, Control, Whisker Assay, Quantitative Proteomics, Two Tailed Test